How does biuret reagent work

First, take 3 dry and clean test tubes. Now add 1 or 2 mL of the test solution, albumin and deionised water in the test tubes. Add Biuret reagent mL in each test tube. Now shake the solution well and let it stand for 5 minutes. Finally, observe how the colour changes. Often ammonium and magnesium ions hinder this test. However, using excess alkali, it can be removed.

No change in colour solution remains blue. Proteins are unavailable. Solution turns violet from blue. Proteins are available. Solution turns pink from blue. Peptides are available.

Positive Result. Colour turns purple. All proteins and peptides give positive. Only amino acid, Histidine, gives a positive result. Negative Result. No change in colour. Following this way, primarily proteins are detected in the concentrations between 0. On the other hand, Molybdenum blue can bind a few specific organic dyes such as malachite green and Auramine o. It results in the further amplification of the signal.

It allows detecting of the proteins with a range of 0. This is also referred to as "Pierce assay" honouring a reagent kit manufacturer.

This method is the simplest and a rapid way to detect protein in a sample. Also, it is less expensive than the Kjedahl test. It provides a stable colour; hence, it does not cause deviations like other methods like UV absorption, Folin-Lowry, etc. Apart from protein, very few compounds interfere in the test.

Proteins can be detected through the use of the Biuret test. Long polypeptides proteins have many peptide bonds and produce a positive reaction to the reagent. The violet color is a positive test for the presence of protein, and the intensity of the color is proportional to the number of peptide bonds in the solution. Proteins are Polymers of Amino Acids Proteins provide much of the structural and functional capacity of cells.

Three amino acids bound into a tripeptide. How Amino Acids Interact with Each Other and the Environment Use the following simulation to test how a polypeptide chain with fold based on the type of solution it is in and the composition of the amino acids.

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